Kinetics investigation of cell growth, xanthan production and sugar cane molasses consumption by Xanthomonas campestris

Pazouki, Mohammad; Zakeri, Ali; Vossoughi, Manoucher

Document Type : Full article

Authors

¹ Materials and Energy Center

² Material and energy research center

³ Chemical and Petroleum Engineering Department, Sharif University of Technology

Abstract

In this research, unstructured kinetic modeling for cell growth evaluation by X.campestris, xanthan production and sugar cane molasses consumption in a batch culture were investigated. Logistic model for biomass growth, Luedeking-Piret model for xanthan biopolymer production and modified Luedeking-Piret model for sugar cane molasses consumption provides an accurate prediction of the fermentation kinetics parameters with high coefficient of determination R2 values.Luedeking-Piret model for xanthan biopolymer production in three different concentration of sugar cane molasses (30, 60 and 90 g/l) as the sole carbon source substrate were studied. A good agreement between experimental and predicted values indicated that the unstructured models were able to describe this fermentation process successfully. The values of specific growth rate μ_max of logestic model for sugar cane molasses (30, 60 and 90 g/l) were 0.029, 0.031 and 0.032 h-1 respectively. The values of α and β is 5.280, 6.594, 8.518 and 0.072, 0.066, 0.086 respectively which shows that the xanthan production is growth associated since the value of the growth associated parameter α is much more than the value of nongrowth associated parameter β in Luedeking Piret model. Moreover, the values of γ and 𝜂 in modified Luedeking-Piret model were obtained.

Keywords

20.1001.1.17355397.2015.12.4.6.8

Main Subjects

Reaction Engineering, Kinetics and Catalysts,

References

[1] Rosalam, S. and England, R., "Review of xanthan gum production from unmodified starches by Xanthomonas campestris sp", Enzyme Microb Tech., 39 (2), 197 (2006).

[2] Becker, A. Katzan, F. Puhler, A. and Ielpi, L., "Xanthan gum biosynthesis and application:a biochemical/genetic perspective ", Appl. Microb. Biotechnol., 50,145 (1998).

[3] Katzbauer, B., "Properties and applications of xanthan gum", Polym. Degrad. Stabil., 59, 81 (1998).

[4] Kwon, G. S. Moon, S. H. Hong, S. D. Lee, H. M. Mheen, T. I. and Oh, H. M., "Rheological properties of extracellular polysaccharide, pestan, produced by pestalotiopsis SP", Biotechnol. Lett., 18, 1465 (1996).

[5] Luong, J. H. T. Mulchandani, A. and Leduy, A., "Kinetics of biopolymers synthesis: a revisit", Enzyme Microb. Tech., 10, 326 (1998).

[6] Garcia-Ochoa, F. Santos, V. E. and Alcon, A., "Xanthan gum production: an unstructured kinetic model", Enzyme Microb. Tech., 17, 206 (1995).

[7] Garcia-Ochoa, F. Santos, V. E. and Alcon, A., "Metabolic structured kinetic model for xanthan gum production", Enzyme Microb. Tech., 23, 75 (1998).

[8] Garcia-Ochoa, F. Santos, V. E. and Alcon, A., "Chemical structured kinetic model for xanthan gum production", Enzyme Microb. Tech., 35, 284 (2004).

[9] Moraine, R. A. and Rogovin, P., "Xanthan biopolymer production at increased concentration by pH control", Biotechnol. Bioeng., 13, 381 (1971).

[10] Basaran-Kurbanoglu, E. and Izzet-Kurbanoglu, N., "Ram horn hydrolysate as enhancer of xanthan production in batch culture of Xanthomonas campestris EBK-4 isolate", Process Biochem., 42, 1146 (2007).

[11] Nakajima, S. Funahashi, H. and Yoshida, T., "Xanthan gum production in a fermenter with twin impellers", Journal of Fermentation and Bioengineering, 70 (6), 392 (1990).

[12] Souw, P. and Demain, A. L., "Nutritional studies on xanthan production by Xanthomonas campestris NRRL B-1459", Appl. Environ. Microbiol., 37, 1186 (1979).

[13] Serrano-Carren, L. Corona, R. M. Sanchez, A. and Galindo, E., "Prediction of xanthan fermentation development by a model linking kinetics, power drawn and mixing", Process Biochem., 33, 133 (1998).

[14] Moosavi, A. and Karbassi, A., "Bioconversion of sugar-beet molasses into xanthan gum", J. Food Process Pres., 34, 316 (2010).

[15] Papagianni, M. Psomas, S. K. Batsilas, L. Paras, S. V. Kyriakidis, A. and Liakopoulou-Kyriakides, M., "Xanthan production by Xanthomonas campestris in batch cultures", Process Biochem., 37, 73 (2001).

[16] Moreno, J. Lopez, C. Vargas-Garcı, M. J. and Vazquez, R., "Use of agricultural wastes for xanthan production by Xanthomonas campestris", J. Ind. Microbiol. Biot., 21, 242 (1998).

[17] Miller, G. L., "Use of Dinitrosalicylic AcidReagent for Determination of Reducing Sugar", Anal. Chem., 31, 426 (1959).

[18] Wang, X. Xu, P. Yuan,Y. Liu, C. and Zhang, D., "Modeling for gellan gum production by Sphingomonas paucimobilis ATCC 31461 in a simplified medium", Appl. Environ. Microbiol., 72 (5), 3367 (2006).

[19] Ozadali, F. and Ozilgen, M., "Microbial growth kinetics of fed-batch fermentations", Appl. Microbiol. Biotechnol., 29, 203 (1988).

[20] Krishna-Ganduri, V. S. R., "Unstructured modeling of aureobasidium pullulans fermentation for pullulan production mathematical approach", Int. J. Eng. Res. Technol., 3, 1076 (2014).

[21] Rajendran, A. and Thangavelu, V., "Application of Central Composite Design and Artificial Neural Network for the Optimization of Fermentation Conditions for Lipase Production by Rhizopus arrhizus MTCC 2233", J. Bioproces. Biotechniq., 2 (3), 1 (2012).

[22] Weiss, R. M. and Ollis, D. F., "Extracellular microbial polysaccharides I.Substrate, biomass and product kinetic equations for batch Xanthan gum fermentation", Biotechnol. Bioeng., 22, 859 (1980).

[23] Pinches, A. and Pallent, L. J., "Rate and yield relationships in the production of xanthan gum by batch fermentations using complex and chemically defined growth media", Biorech. Bioeng., 28, 1484 (1986).

Iranian Journal of Chemical Engineering(IJChE)

Kinetics investigation of cell growth, xanthan production and sugar cane molasses consumption by Xanthomonas campestris

References

References

Volume 12, Issue 4 - Serial Number 4
October 2015
Pages 84-92

Kinetics investigation of cell growth, xanthan production and sugar cane molasses consumption by Xanthomonas campestris

References

References

Volume 12, Issue 4 - Serial Number 4October 2015Pages 84-92

Volume 12, Issue 4 - Serial Number 4
October 2015
Pages 84-92